MSC Secretome's Antibacterial Activity on P. aeruginosa Isolated from Diabetic Ulcer Patient

  • Dini Assyfa INA Lab Padang
  • Marlina Marlina Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia
  • Akmal Djamaan Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia

Abstract

The study aims to determine the antibacterial activity of adipose tissue-derived Mesenchymal Stem Cell (MSC) secretome against P. aeruginosa bacteria isolated from diabetic ulcer patients. Bacteria were isolated from specimens obtained from diabetic ulcer patient and identified using MacConkey and Blood Agar media followed by Polymerase Chain Reaction (PCR) molecular identification. Confirmed P. aeruginosa bacteria were used to evaluate the MSC secretome's antibacterial activity via the Kirby-Bauer method. Isolated bacteria grew on MacConkey media as gram-negative, non-lactose-fermenting bacteria, showing hemolytic capabilities on Blood Agar media. PCR identification yielded positive results for P. aeruginosa. In antibacterial activity testing against isolated bacteria, MSC secretome at concentrations of 1.25%, 2.5%, 5%, and 10% exhibited efficacy with average inhibitory zones measuring 8.17 mm, 8.23 mm, 8.52 mm, and 9.30 mm, respectively. The MSC secretome demonstrates antibacterial activity against P. aeruginosa bacteria isolated from diabetic ulcer patients.

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Keywords: MSC secretome, diabetic ulcer, P.aeruginosa, Antibacterial activity

References

Al-Ahmadi, J. and Roodsari, Z. (2016) ‘FAST AND SPECIFIC DETECTION OF PSEUDOMONAS AERUGINOSA FROM OTHER PSEUDOMONAS SPECIES BY PCR’, Annals of Burns and Fire Disasters , 29(4), pp. 264–267.
Alavi, A. et al. (2014) ‘Diabetic foot ulcers: Part I. Pathophysiology and prevention’, Journal of the American Academy of Dermatology. Mosby Inc., pp. 1.e1-1.e18. Available at: https://doi.org/10.1016/j.jaad.2013.06.055.
Alcayaga-Miranda, F., Cuenca, J. and Khoury, M. (2017) ‘Antimicrobial activity of mesenchymal stem cells: Current status and new perspectives of antimicrobial peptide-based therapies’, Frontiers in Immunology. Frontiers Media S.A. Available at: https://doi.org/10.3389/fimmu.2017.00339.
Allen, M.E. (2016) ‘MacConkey Agar Plates Protocols’. American Society For Microbiology.
Bahroudi, M. et al. (2020) ‘Antibacterial and antibiofilm activity of bone marrow-derived human mesenchymal stem cells secretome against Vibrio cholerae’, Microbial Pathogenesis, 139. Available at: https://doi.org/10.1016/j.micpath.2019.103867.
Buxton, R. (2016) ‘Blood Agar Plates and Hemolysis Protocols’. Available at: www.asmscience.org.
Ertugrul, B.M. et al. (2017) ‘Risk Factors for Infection with Pseudomonas aeruginosa in Diabetic Foot Infections’, Journal of the American Podiatric Medical Association, 107(6), pp. 483–489.
Hudzicki, J. (2016) Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. American Society for Microbiology. Available at: www.atcc.org.
Khanabdali, R. et al. (2016) ‘Harnessing the secretome of cardiac stem cells as therapy for ischemic heart disease’, Biochemical Pharmacology. Elsevier Inc., pp. 1–11. Available at: https://doi.org/10.1016/j.bcp.2016.02.012.
Kunz Coyne, A.J. et al. (2022) ‘Therapeutic Strategies for Emerging Multidrug-Resistant Pseudomonas aeruginosa’, Infectious Diseases and Therapy. Adis, pp. 661–682. Available at: https://doi.org/10.1007/s40121-022-00591-2.
Pachori, P., Gothalwal, R. and Gandhi, P. (2019) ‘Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review’, Genes and Diseases. Chongqing University, pp. 109–119. Available at: https://doi.org/10.1016/j.gendis.2019.04.001.
Sankar, N., Moinuddin, S.K. and Mohan, S. (2020) ‘Microbial etiology of diabetic foot ulcers: Swab versus tissue culture’, International Journal of Surgery Science, 4(2), pp. 146–148. Available at: https://doi.org/10.33545/surgery.2020.v4.i2c.408.
Smith, A.C. and Hussey, M.A. (2019) Gram Stain Protocols. Available at: www.asmscience.org.
Sultana, R. et al. (2023) ‘Diabetic foot ulcer-a systematic review on relevant microbial etiology and antibiotic resistance in Asian countries’, Diabetes & Metabolic Syndrome: Clinical Research & Reviews , 17(6).
Talapko, J. et al. (2022) ‘Antimicrobial Peptides—Mechanisms of Action, Antimicrobial Effects and Clinical Applications’, Antibiotics. MDPI. Available at: https://doi.org/10.3390/antibiotics11101417.
Published
2024-06-30
How to Cite
Assyfa, D., Marlina, M., & Djamaan, A. (2024). MSC Secretome’s Antibacterial Activity on P. aeruginosa Isolated from Diabetic Ulcer Patient. JOPS (Journal Of Pharmacy and Science), 7(2), 1-9. https://doi.org/10.36341/jops.v7i2.4717
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